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Handijatno, Didik
- Compare Transferrin Binding Protein (Tbp A) Gene Virulence of Pasteurella Multocida Type B of Buffalo and Vaccine Septicemiaepizootica
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Authors
Affiliations
1 Airlangga University, Jl. Mulyorejo, Kampus C Unair, Surabaya, ID
1 Airlangga University, Jl. Mulyorejo, Kampus C Unair, Surabaya, ID
Source
Indian Journal of Public Health Research & Development, Vol 10, No 12 (2019), Pagination: 1640-1644Abstract
Septicemia Epizootica (SE), is a fatal acute septicemia disease by Pasteurella multocida. B:2,5 and E:2,5 in cattle and buffalo. TbpA has a number of important role for bacterial cells, such as nutrient uptake, transport of molecules in and out of cells and interactions with the environment and host. P. multocida iron acquisition related gene, tbpA, has been considered as an important epidemiological marker. The purpose of this study was to compare the Transferrin Binding Protein A (TbpA) gene from P. multocidatype B of buffalo and it with vaccine strain. Characterization of TbpA of P. multocida and vaccine strain was done using PCR then sequencing. Sample used in this study were P. multocida local isolate from NTT and vaccine strain (Katha). Isolation and identification were performed using bacteriological culture and biochemically characterization. Results from PCR showed positive a tbpA gene on P. multocida local isolate and vaccine strainis. The comparison of the results of sequencing between the tbpa gene isolates P.multocida and vaccines showed deletion.Keywords
Septisemiaepizootica, Pasteurellamultocida Type B, Transferrin Binding Protein A (TbpA) Gene, PCR, Buffalo.- Sequence Homology and Epitope Prediction of 37 kDa Outer Membrane Protein H(ompH) Gene of Pasteurella Multocida Type B Isolate from Nusa Tenggara Timur (NTT)
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Authors
Firdausy Kurnia Maulana
1,
Didik Handijatno
2,
Hani Plumeriastuti
3,
Rahaju Ernawati
2,
Wiwiek Tyasningsih
2,
Mufasirin
4
Affiliations
1 Airlangga University, ID
2 Department of Microbiology and Mycology, Airlangga University, ID
3 Department of Veterinary Pathology, Airlangga University, ID
4 Department of Veterinary Parasitology, Airlangga University, ID
1 Airlangga University, ID
2 Department of Microbiology and Mycology, Airlangga University, ID
3 Department of Veterinary Pathology, Airlangga University, ID
4 Department of Veterinary Parasitology, Airlangga University, ID